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GrainGenes Reference Report: TPJ-30-83

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Reference	TPJ-30-83
Title	cDNA microarray analysis of gene expression during Fe-deficiency stress in barley suggests that polar transport of vesicles is implicated in phytosiderophore secretion in Fe-deficient barley roots
Journal	Plant Journal
Year	2002
Volume	30
Pages	83-94
Author	Negishi T [ Show all 12 ]
Abstract	Summary: To acquire Fe from soil, graminaceous plants secrete mugineic acid family phytosiderophores (MAs) from their roots. The secretion of MAs increases in response to Fe deficiency, and shows a distinct diurnal rhythm. We used a microarray that included 8987 cDNAs of rice EST clones to examine gene expression profiles in barley roots during Fe-deficiency stress. Approximately 200 clones were identified as Fe-deficiency-inducible genes, of which seven had been identified previously. In order to meet the increased demand for methionine to produce MAs, Fe-deficiency enhances the expression of genes that participate in methionine synthesis, as well as recycling methionine through the Yang cycle. Of these 200 genes, approximately 50 exhibited different transcription levels in Fe-deficient roots at noon and at night. Northern blot analysis of time course experiments confirmed that five of these genes exhibited a diurnal change in their level of expression. The diurnal changes in the expression of these genes suggest that polar vesicle transport is involved in the diurnal secretion of MAs
External Databases	Pubmed: 11967095
Keyword	[ Hide all but 1 of 51 ] 2'-deoxymugineic acid 50 acid arabidopsis barley barley roots binding complex biosynthesis cdna cdna microarray clone cycle deficiency demand diurnal change diurnal rhythm est family family phytosiderophores fe-deficiency gene gene expression gene expression profiles graminaceous plants hordeum vulgare increase iron-deficiency mas methionine methionine synthesis microarray microarray analysis mugineic acid mugineic acid family phytosiderophores northern northern blot order phytosiderophore polar transport profile rice root secretion soil stress synthesis time-course transcription transport vesicle transport vesicles

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