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GrainGenes Reference Report: TPJ-29-453

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Reference	TPJ-29-453
Title	The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm-specific genes during seed development
Journal	Plant Journal
Year	2002
Volume	29
Pages	453-464
Author	Diaz I [ Show all 6 ]
Abstract	Summary: HvGAMYB, a MYB transcription factor previously shown to be expressed in barley aleurone cells in response to gibberellin during germination, also has an important role in gene regulation during endosperm development. The mRNA was detected early (10 DAF) in the seeds where it accumulates, not only in the aleurone layer, starchy endosperm, nucellar projection and vascular tissue, but also in the immature embryo as shown by in situ hybridization analysis. The HvGAMYB protein, expressed in bacteria, binds to oligonucleotides containing the 5'-TAACAAC-3' or 5'-CAACTAAC-3' sequences, derived from the promoter regions of the endosperm-specific genes Hor2 and Itr1, encoding a B-hordein and trypsin-inhibitor BTI-CMe, respectively. Binding is prevented when these motifs are mutated to 5'- TgACAAg-3' and 5'-CgACTgAC-3'. Transient expression experiments in co-bombarded developing endosperms demonstrate that HvGAMYB trans-activates transcription from native Hor2 and Itr1 promoters through binding to the intact motifs described above. Trans-activation of the Hor2 promoter also requires an intact prolamine box (PB). This suggests that HvGAMYB interacts in developing barley endosperms with the PB-binding factor BPBF, an endosperm-specific DOF transcriptional activator of the Hor2 gene. The in vivo interaction experiment between HvGAMYB and BPBF was done in the yeast two-hybrid system, where HvGAMYB potentiates the BPBF trans-activation capacity through interaction with its C-terminal domain
External Databases	Pubmed: 11846878
Keyword	[ Hide all but 1 of 73 ] acting elements activator aleurone aleurone cells aleurone layer b-hordein bacteria barley barley aleurone barley aleurone cells barley developing endosperm barley endosperm binds box bzip protein capacity cell developing barley endosperm dof protein domain embryo endosperm endosperm development gamyb gamyb protein gcn4-like motif gene gene regulation germination gibberellin hor2 promoter hybridization immature embryo in situ in situ hybridization in vivo interacts itr1 promoter layer motif mrna myb oligonucleotides pb prolamin genes promoter protein region regulation regulatory elements rice glutelin gene seed seed development sequence situ hybridization starchy endosperm system tissue transactivation transcription transcription factor transcriptional activator transient transient expression trypsin inhibitor two-hybrid two-hybrid system vascular tissue vivo yeast yeast two-hybrid yeast two-hybrid system zein gene

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