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GrainGenes Reference Report: TPJ-25-335

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Reference	TPJ-25-335
Title	The MLA6 coiled-coil, NBS-LRR protein confers AvrMla6-dependent resistance specificity to Blumeria graminis f. sp. hordei in barley and wheat
Journal	The Plant Journal
Year	2001
Volume	25
Pages	335-348
Author	Halterman D [ Show all 7 ]
Abstract	Summary: The barley Mla locus confers multiple resistance specificities to the obligate fungal biotroph, Blumeria (= Erysiphe) graminis f. sp. hordei. Interspersed within the 240 kb Mla complex are three families of resistance gene homologs (RGHs). Probes from the MIa-RGH1 family were used to identify three classes of cDNAs. The first class is predicted to encode a full-length CC-NBS-LRR protein and the other two classes contain alternatively spliced, truncated variants. Utilizing a cosmid that contains a gene corresponding to the full-length candidate cDNA, two single-cell expression assays were used to demonstrate complementation of AvrMla6-dependent, resistance specificity to B. graminis in barley and wheat. The first of these assays was also used to substantiate previous genetic data that the Mla6 allele requires the signaling pathway component, Rar1, for function. Computational analysis of MLA6 and the Rar1-independent, MLA1 protein reveals 91.2% identity and shows that the LRR domain is subject to diversifying selection. Our findings demonstrate that highly related CC-NBS-LRR proteins encoded by alleles of the Mla locus can dictate similar powdery mildew resistance phenotypes yet still require distinct downstream signaling components
Gene	Mla6 (Hordeum)
Sequence	AJ302292
Keyword	[ Hide all but 1 of 64 ] 5' untranslated region alleles allelic variation alternative splicing amino acid sequence assay assays barley blumeria graminis cc-nbs-lrr cdna class-i coiled-coil complementary dna complementation complexes component cosmids disease resistance divergent selection domain erysiphe erysiphe graminis erysiphe graminis f family flax rust resistance function fungal gene gene expression gene-for-gene concept genetic graminis heterologous resistance specificity homolog hordei hordeum vulgare loci locus lrr mildew mildew resistance multigene family multiple nbs lrr nucleotide sequence pathway phenotype phenotypes plant proteins powdery mildew powdery mildew resistance probes protein rar1-dependent signaling resistance resistance gene selection sp sp hordei specificity tobacco mosaic-virus triticum aestivum variant

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