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GrainGenes Reference Report: TPJ-13-13

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Reference	TPJ-13-13
Title	In vitro interactions between barley TALE homeodomain proteins suggest a role for protein-protein associations in the regulation of Knox gene function
Journal	The Plant Journal
Year	2001
Pages	13-23
Author	Muller J [ Show all 6 ]
Abstract	Summary: This paper describes two-hybrid interactions amongst barley homeodomain proteins encoded by the Three Amino acid Loop Extension (TALE) superfamily. The class I KNOX protein BKN3 is shown to homodimerise and to associate with proteins encoded by the class I and II Knox genes BKn-1 and BKn-7. Furthermore, JUBEL1 and JUBEL2, two BELL1 homologous proteins, are identified and characterised as interacting partners of BKN3. Differences in the requirements of BKN3 derivatives for interactions with KNOX and JUBEL proteins imply the involvement of overlapping but slightly different domains. This set of results is an example for interactions amongst different classes of plant TALE homeodomain proteins, as previously described for related animal proteins. Apparently identical spatial and temporal expression patterns of BKn-1, BKn-3, BKn-7, JuBel1 and JuBel2, as determined by in situ hybridisation, are compatible with possible interactions of their protein products in planta. Contradictory to the common model, that the transcriptional down-regulation of certain class 1 Knox-genes is the prerequisite for organ differentiation, transcripts of all five genes were, similar to Tkn1 and Tkn2/LeT6 of tomato, detected in incipient and immature leaves as well as in meristematic tissues. A characteristic phenotype is induced by the overexpression of JuBel2 in transgenic tobacco plants
Keyword	[ Hide all but 1 of 52 ] amino acid amino acid sequence animal proteins associations barley class-i derivatives differentiation dna hybridization down regulation expression patterns extension gene gene interaction genetic regulation homeodomain homeodomain protein homologous proteins hordeum vulgare in situ in vitro involvement knox genes meristem model mutant nicotiana tabacum nucleotide sequence overexpression paper pattern phenotype prerequisite products regulation spatial variation superfamily tale homeodomain proteins temporal variation tissue tobacco tobacco plants tomato transcript transcription transcription factors transgenic transgenic plant transgenic tobacco transgenic tobacco plants two-hybrid vitro

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