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GrainGenes Reference Report: TIB-13-324

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Reference	TIB-13-324
Title	Gene-transfer and plant--regeneration techniques
Journal	Trends in Biotechnology (Tibtech)
Year	1995
Volume	13
Pages	324-331
Author	Walden R Wingender R
Abstract	The production of transgenic plants depends on the stable introduction of foreign DNA into the plant genome, followed by regeneration to produce intact plants, and the subsequent expression of the introduced gene(s) A variety of transformation methods have been developed, and this has allowed many of the world's important crop plants to be transformed Transformation mediated by Agrobacterium has provided a reliable means of creating transgenics in a wide variety of species that are amenable to tissue culture and regeneration Vectorless gene-transfer systems include particle bombardment (biolistics), electroporation and membrane permeabilization using chemicals Of these, biolistics has proved to be particularly successful with plants that are less amenable to tissue culture, such as cereals and legumes.
Keyword	[ Hide all but 1 of 10 ] agrobacterium-tumefaciens biolistic-transformation crops direct-dna-uptake electroporation gene transfer genetic transformation literature-reviews plants transgenic-plants

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