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GrainGenes Reference Report: TGR-12-587

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Reference	TGR-12-587
Title	Generation of rye (Secale cereale L.) plants with low transgene copy number after biolistic gene transfer and production of instantly marker-free transgenic rye
Journal	Transgenic Research
Year	2003
Volume	12
Pages	587-596
Author	Popelka JC Xu JP Altpeter F
Keyword	[ Hide all but 1 of 57 ] agent agents agrobacterium amplification bar biolistic parameters callus cereale l copy number copy-number dna delivery efficient frequencies gene gene silencing generation genetic transformation genetic-transformation high frequency high-frequency impact integration integration patterns marker marker gene-free multiple parameters particle bombardment pcr pcr amplification progeny protocol regeneration rye rye (secale cereale l.) safety secale selectable marker selectable markers selectable-marker-free selection selective agents sequence sexual stable transformation t-dna tissue tissue culture transfer transformation transgene transgene copy number transgene expression transgene integration transgene pyramiding transgenic wheat triticum-aestivum

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