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GrainGenes Reference Report: TAG-115-301

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Reference	TAG-115-301
Title	An integrated molecular linkage map of diploid wheat based on a Triticum boeoticum x T. monococcum RIL population
Journal	Theoretical and Applied Genetics
Year	2007
Volume	115
Pages	301-312
Author	[ Hide all but 1 of 8 ] Singh K Ghai M Garg M Chhuneja P Kaur P Schnurbusch T Keller B Dhaliwal HS
Abstract	Diploid A genome species of wheat harbour immense variability for biotic stresses and productivity traits, and these could be transferred efficiently to hexaploid wheat through marker assisted selection, provided the target genes are tagged at diploid level first. Here we report an integrated molecular linkage map of A genome diploid wheat based on 93 recombinant inbred lines (RILs) derived from Triticum boeoticum x Triticum monococcum inter sub-specific cross. The parental lines were analysed with 306 simple sequence repeat (SSR) and 194 RFLP markers, including 66 bin mapped ESTs. Out of 306 SSRs tested for polymorphism, 74 (24.2%) did not show amplification (null) in both the parents. Overall, 171 (73.7%) of the 232 remaining SSR and 98 (50.5%) of the 194 RFLP markers were polymorphic. Both A and D genome specific SSR markers showed similar transferability to A genome of diploid wheat species. The 176 polymorphic markers, that were assayed on a set of 93 RILs, yielded 188 polymorphic loci and 177 of these as well as two additional morphological traits mapped on seven linkage groups with a total map length of 1,262 cM, which is longer than most of the available A genome linkage maps in diploid and hexaploid wheat. About 58 loci showed distorted segregation with majority of these mapping on chromosome 2Am. With a few exceptions, the position and order of the markers was similar to the ones in other maps of the wheat A genome. Chromosome 1Am of T. monococcum and T. boeoticum showed a small paracentric inversion relative to the A genome of hexaploid wheat. The described linkage map could be useful for gene tagging, marker assisted gene introgression from diploid into hexaploid wheat as well as for map based cloning of genes from diploid A genome species and orthologous genes from hexaploid wheat.
External Databases	https://doi.org/10.1007/s00122-007-0543-z
Gene	Rc-A1 (Triticum)
Germplasm	Triticum boeoticum pau5088
Map Data	T. boeoticum x monococcum
Image	T. boeoticum x T. monococcum map

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