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GrainGenes Reference Report: TAG-109-911

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Reference	TAG-109-911
Title	Reduced metaphase I associations between the short arms of homoeologous chromosomes in a Hordeum vulgare L. x H. bulbosum L. diploid hybrid influences the frequency of recombinant progeny
Journal	Theoretical and Applied Genetics
Year	2004
Volume	109
Pages	911-916
Author	Pickering RA Hudakova S Houben A Johnston PA Butler RC
Abstract	Hordeum vulgare (cultivated barley) x H. bulbosum (bulbous barley grass) hybrids have been used to obtain disease-resistant recombinant lines (RLs). The RLs contain chromatin transferred from the wild species mostly onto the long arms of recipient barley chromosomes. To determine whether differences in meiotic metaphase I (MI) associations between the long and short arms of homoeologous chromosomes can account for the preponderance of introgressions on the long arms, we carried out fluorescent in situ hybridisation on MI chromosome preparations obtained from pollen mother cells of a diploid interspecific hybrid. By using various probes, we established that MI associations between the long arms occurred more frequently than between the short arms for the five chromosomes tested
Keyword	[ Hide all but 1 of 17 ] barley cells chromatin chromosome pairing chromosomes fluorescence in situ hybridization genetic recombination Hordeum bulbosum Hordeum vulgare hybrids interspecific hybridization introgression lines meiosis metaphase pollen progeny

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