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GrainGenes Reference Report: TAG-109-847

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Reference	TAG-109-847
Title	Isolation of gibberellin metabolic pathway genes from barley and comparative mapping in barley, wheat and rice
Journal	Theoretical and Applied Genetics
Volume	109
Pages	847-855
Author	Spielmeyer W [ Show all 6 ]
Abstract	Gene sequences encoding gibberellin (GA) biosynthetic and catabolic enzymes were isolated from Himalaya barley. These genes account for most of the enzymes required for the core pathway of GA biosynthesis as well as for the first major catabolic enzyme. By means of DNA gel blot analysis, we mapped coding sequences to chromosome arms in barley and wheat using barley-wheat chromosome addition lines, nulli-tetrasomic substitution and ditelosomic lines of wheat. These same sequences were used to identify closely related sequences from rice, which were mapped in silico, thereby allowing their syntenic relationship with map locations in barley and wheat to be investigated. Determination of the chromosome arm locations for GA metabolic genes provides a framework for future studies investigating possible identity between GA metabolic genes and dwarfing genes in barley and wheat
Keyword	[ Hide all but 1 of 21 ] amino acid sequences barley biosynthesis complementary dna dna probes dwarfing enzymes genes gibberellins hordeum vulgare metabolism molecular sequence data nucleotide sequences oxidoreductases oxygenases physical chromosome mapping rice rye secale cereale triticum aestivum wheat

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