GrainGenes Reference Report: TAG-109-847
Reference
TAG-109-847
Title
Isolation of gibberellin metabolic pathway genes from barley and comparative mapping in barley, wheat and rice
Journal
Theoretical and Applied Genetics
Volume
109
Pages
847-855
Author
[ Hide all but 1 of 6 ] Spielmeyer W Ellis M Robertson M Ali S Lenton JR Chandler PM
Abstract
Gene sequences encoding gibberellin (GA) biosynthetic and catabolic enzymes were isolated from Himalaya barley. These genes account for most of the enzymes required for the core pathway of GA biosynthesis as well as for the first major catabolic enzyme. By means of DNA gel blot analysis, we mapped coding sequences to chromosome arms in barley and wheat using barley-wheat chromosome addition lines, nulli-tetrasomic substitution and ditelosomic lines of wheat. These same sequences were used to identify closely related sequences from rice, which were mapped in silico, thereby allowing their syntenic relationship with map locations in barley and wheat to be investigated. Determination of the chromosome arm locations for GA metabolic genes provides a framework for future studies investigating possible identity between GA metabolic genes and dwarfing genes in barley and wheat
Keyword
amino acid sequences
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-109-847
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||