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GrainGenes Reference Report: TAG-109-713

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Reference	TAG-109-713
Title	Isolation and mapping of resistance gene analogs from the Avena strigosa genome
Journal	Theoretical and Applied Genetics
Volume	109
Pages	713-724
Author	Irigoyen ML Loarce Y Fominaya A Ferrer E
Abstract	Degenerate primers based on conserved regions of the nucleotide binding site (NBS) domain (encoded by the largest group of cloned plant disease resistance genes) were used to isolate a set of 15 resistance gene analogs (RGA) from the diploid species Avena strigosa Schreb. These were grouped into seven classes on the basis of 60% or greater nucleic acid sequence identity. Representative clones were used for genetic mapping in diploid and hexaploid oats. Two RGAs were mapped at two loci of the linkage group AswBF belonging to the A. strigosa x A. wiestii Steud map, and ten RGAs were mapped at 15 loci in eight linkage groups belonging to the A. byzantina C. Koch cv. Kanota x A. sativa L. cv. Ogle map. A similar approach was used for targeting genes encoding receptor-like kinases. Three different sequences were obtained and mapped to two linkage groups of the hexaploid oat map. Associations were explored between already known disease resistance loci mapped in different populations and the RGAs. Molecular markers previously linked to crown rust and barley yellow dwarf resistance genes or quantitative trait loci were found in the Kanota x Ogle map linked to RGAs at a distance ranging from 0 cM to 20 cM. Homoeologous RGAs were found to be linked to loci either conferring resistance to different isolates of the same pathogen or to different pathogens. This suggests that these RGAs identify genome regions containing resistance gene clusters
External Databases	https://doi.org/10.1007/s00122-004-1679-8
Locus	II2.3 [ Show all 23 ]
Map Data	Oat-2004-SxW-RGA Oat-2004-KxO-RGA
Probe	II2.17 [ Show all 12 ]
Keyword	[ Hide all but 1 of 26 ] amino acid sequences avena sativa avena strigosa avena wiestii barley barley yellow dwarf virus chromosome mapping cloning (molecular) disease resistance genes genetic markers genetic resistance genome interspecific hybridization linkage groups loci molecular sequence data nucleotide sequences plant pathogenic fungi plant viruses puccinia coronata quantitative trait loci restriction fragment length polymorphism rust diseases sequence alignment wild relatives

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