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GrainGenes Reference Report: TAG-109-210

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Reference	TAG-109-210
Title	Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL
Journal	Theoretical and Applied Genetics
Year	2004
Volume	109
Pages	210-214
Author	Singrun C Hsam SLK Zeller FJ Wenzel G Mohler V
Abstract	Summary: Segregation analysis of resistance to powdery mildew in a F2 progeny from the cross Chinese Spring (CS) x TA2682c revealed the inheritance of a dominant and a recessive powdery mildew resistance gene. Selfing of susceptible F2 individuals allowed the establishment of a mapping population segregating exclusively for the recessive resistance gene. The extracted resistant derivative showing full resistance to each of 11 wheat powdery mildew isolates was designated RD30. Amplified fragment length polymorphism (AFLP) analysis of bulked segregants from F3s showing the homozygous susceptible and resistant phenotypes revealed an AFLP marker that was associated with the recessive resistance gene in repulsion phase. Following the assignment of this AFLP marker to wheat chromosome 7A by means of CS nullitetrasomics, an inspection of simple sequence repeat (SSR) loci evenly spaced along chromosome 7A showed that the recessive resistance gene maps to the distal region of chromosome 7AL. On the basis of its close linkage to the Pm1 locus, as inferred from connecting partial genetic maps of 7AL of populations CS x TA2682c and CS x Virest (Pm1e), and its unique disease response pattern, the recessive resistance gene in RD30 was considered to be novel and tentatively designated mlRD30
External Databases	Pubmed: 15014874
Gene	mlRd30 (Triticum)
Locus	mlRd30
Keyword	[ Hide all but 1 of 39 ] aflp amplified fragment length polymorphism chinese chromosome common wheat disease disease resistance establishment f2 gene hope inheritance isolate leaf rust resistance linkage localization map mapping mildew mildew resistance pattern phenotype pm1 polymorphism powdery mildew powdery mildew resistance repeat resistance resistance gene resistant segregation selfing sequence simple sequence repeat ssr susceptible triticum aestivum unique wheat powdery mildew

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