GrainGenes Reference Report: TAG-109-55
Reference
TAG-109-55
Title
Identification of Canadian durum wheat varieties using a single PCR
Journal
Theoretical and Applied Genetics
Year
2004
Volume
109
Pages
55-61
Author
Perry DJ
Abstract
Summary: Accurate and reliable means of variety identification are necessary to assess purity of seed supplies, to support claims relating to plant breeders' rights and, in Canada, to provide quality assurances in the grain handling system. A single, multiplexed set of seven simple-sequence-repeat (SSR) markers was found to uniquely identify all 18 durum wheat varieties that have been developed in Canada and are currently, or were formerly, registered for commercial production. Significant features of this multiplexed set include an allele that is specific, within Canadian durum varieties, to those having high gluten strength, and redundancy that was included in an effort to increase the capacity to accommodate future varieties. In combination with a reasonably rapid individual-kernel DNA extraction protocol and automated allele calling, this marker system offers a higher resolution alternative to complement established protein-based variety identification methods
External Databases
Pubmed: 14985973
Keyword
[ Hide all but 1 of 22 ] allele breeders canada capacity cultivar dna dna extraction durum extraction features gluten identify pcr production quality redundancy seed set simple sequence repeat specific ssr wheat varieties
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-109-55
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||