Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-109-48

[Submit comment/correction]

Reference	TAG-109-48
Title	Genome and species relationships in genus Avena based on RAPD and AFLP molecular markers
Journal	Theoretical and Applied Genetics
Year	2004
Volume	109
Pages	48-54
Author	Drossou A Katsiotis A Leggett JM Loukas M Tsakas S
Abstract	Summary: Species and genome relationships among 11 diploid (A and C genomes), five tetraploid (AB and AC genomes) and two hexaploid (ACD genome) Avena taxa were investigated using amplified fragment length polymorphisms (AFLPs) and random amplified polymorphic DNA (RAPD) markers. The two primer pairs used for the AFLP reactions produced a total of 354 polymorphic bands, while 187 reproducible bands were generated using ten RAPD primers. Genetic similarities amongst the entries were estimated using the Jaccard and Dice algorithms, and cluster analyses were performed using UPGMA and neighbor joining methods. Principle coordinate analysis was also applied. The highest cophenetic correlation coefficient was obtained for the Jaccard algorithm and UPGMA clustering method (r=0.99 for AFLP and r=0.94 for RAPD). No major clustering differences were present between phenograms produced with AFLPs and RAPDs. Furthermore, data produced with AFLPs and RAPDs were highly correlated (r=0.92), indicating the reliability of our results. All A genome diploid taxa are clustered together according to their karyotype. The AB genome tetraploids were found to form a subcluster within the As genome diploids (AFLPs), indicating their near-autoploid origin. The AC genome tetraploids are clustered to the ACD genome hexaploids. Finally, the C genome diploids form an outer branch, indicating the major genomic divergence between the A and C genomes in Avena
External Databases	Pubmed: 15014877
Keyword	[ Hide all but 1 of 32 ] a genome algorithm algorithms amplified fragment length polymorphism amplified polymorphic dna branch c genome cluster difference diploid discrimination divergence dna evolution genetic similarity genetic-relationships genus hexaploid hybrid in situ hybridization karyotype oat cultivars origin polymorphic dna primer random amplified polymorphic dna rapd rapds repetitive dna-sequences species relationships tetraploid tetraploids

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.