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GrainGenes Reference Report: TAG-108-1409

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Reference	TAG-108-1409
Title	Characterisation and marker development for low molecular weight glutenin genes from Glu-A3 alleles of bread wheat (Triticum aestivum. L)
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	1409-1419
Author	Zhang W Gianibelli MC Rampling LR Gale KR
Abstract	Summary: PCR was used to amplify low-molecular-weight (LMW) glutenin genes from the Glu-A3 loci of hexaploid wheat cultivars containing different Glu-A3 alleles. The complete coding sequence of one LMW glutenin gene was obtained for each of the seven alleles Glu-A3a to Glu-A3g. Chromosome assignment of PCR products using Chinese Spring nulli-tetrasomic lines confirmed the amplified products were from chromosome 1A. All sequences were classified as LMW-i-type genes based on the presence of an N-terminal isoleucine residue and eight cysteine residues located within the C-terminal domain of the predicted, mature amino acid sequence. All genes contained a single uninterrupted open reading frame, including the sequence from the Glu-A3e allele, for which no protein product has been identified. Comparison of LMW glutenin gene sequences obtained from different alleles showed a wide range of sequence identity between the genes, with between 1 and 37 single nucleotide polymorphisms and between one and five insertion/deletion events between genes from different alleles. Allele-specific PCR markers were designed based on the DNA polymorphisms identified between the LMW glutenin genes, and these markers were validated against a panel of cultivars containing different Glu-A3 alleles. This collection of markers represents a valuable resource for use in marker-assisted breeding to select for specific alleles of this important quality-determining locus in bread wheat
External Databases	Pubmed: 14727031
Allele	Glu-A3g (Triticum)
Keyword	[ Hide all but 1 of 45 ] allele allele-specific pcr amino amino acid sequence bread breeding c-terminal domain chinese chromosome collection cysteine cysteine residues dna dna polymorphism domain dough properties durum wheat flour quality gene gene sequence gliadin proteins glutenin hexaploid hexaploid wheat nucleotide open reading frame pcr pcr markers pcr products prairie spring wheat products prolamin genes protein sequence single nucleotide polymorphism single nucleotide polymorphisms specific storage protein subunit composition triticum triticum aestivum two-dimensional electrophoresis weight glutenin weight glutenin gene weight glutenin genes

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