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GrainGenes Reference Report: TAG-108-1401

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Reference	TAG-108-1401
Title	High-resolution genetic mapping of the leaf stripe resistance gene Rdg2a in barley
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	1401-1408
Author	Bulgarelli D [ Show all 8 ]
Abstract	Summary: The dominant gene Rdg2a of barley conferring resistance to the hemi-biotrophic seed-borne pathogen Pyrenophora graminea is located in the distal region of chromosome arm 1 (7H)S. As the first step towards isolating the gene, a high-resolution genetic map of the region was constructed using an F2 population of 1,400 plants (ThibautRdg2a x Mirco). The map included six classes of resistance gene analogues (RGAs) tightly associated with Rdg2a. Rdg2a was delimited to a genetic interval of 0.14 cM between the RGAs ssCH4 and MWG851. Additional markers were generated using the sequence from the corresponding region on rice chromosome 6, allowing delimitation of the Rdg2a syntenic interval in rice to a 115 kbp stretch of sequence. Analysis of the rice sequence failed to reveal any genes with similarity to characterized resistance genes. Therefore, either the rice-barley synteny is disrupted in this region, or Rdg2a encodes a novel type of resistance protein
External Databases	Pubmed: 14689188
Keyword	[ Hide all but 1 of 36 ] barley chromosome cm conferring resistance dna dominant gene f-2 population f2 gene genetic map genetic mapping high-resolution homolog hordeum vulgare interval localization map mapping oryza sativa pathogen population protein pyrenophora graminea pyrenophora-graminea resistance resistance gene resistance gene analogue resistance gene analogues reveal rga rice rice genome rpg1 region sequence step synteny

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