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GrainGenes Reference Report: TAG-108-1349

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Reference	TAG-108-1349
Title	Identification and molecular characterization of a novel y-type Glu-D-t 1 glutenin gene of Aegilops tauschii
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	1349-1358
Author	Yan Y [ Show all 9 ]
Abstract	Summary: A novel y-type high-molecular-weight glutenin subunit possessing a slightly faster mobility than that of subunit 1Dy12 in SDS-PAGE, designated 1Dy12.1t in Aegilops tauschi, was identified by one- and two-dimensional gel and capillary electrophoresis. Its coding gene at the Glu-Dt 1 locus was amplified with allele-specific-PCR primers, and the amplified products were cloned and sequenced. The complete nucleotide sequence of 2,807 bp containing an open reading frame of 1,950 bp and 857 bp of upstream sequence was obtained. A perfectly conserved enhancer sequence and the -300 element were present at positions of 209-246 bp and 424-447 bp upstream of the ATG start codon, respectively. The deduced mature protein of 1 Dy12.1t subunit comprised 648 amino acid residues and had a Mr of 67,518 Da, which is slightly smaller than the 1Dy12 (68,695 Da) but larger than the 1Dy10 (67,495 Da) subunits of bread wheat, respectively, and corresponds well with their relative mobilities when separated by acid-PAGE. The deduced amino acid sequence indicated that the 1Dy12.1t subunit displayed a greater similarity to the 1Dy10 subunit, with only seven amino acid substitutions, suggesting that this novel gene could have positive effect on bread-making quality. A phenetic tree produced by nucleotide sequences showed that the x- and y-type subunit genes were respectively clustered together and that the Glu-D t 1y12.1 gene of Ae. tauschii is closely related to other y-type subunit genes from the B and D genomes of hexaploid bread wheat
External Databases	Pubmed: 14740085
Keyword	[ Hide all but 1 of 40 ] acid-polyacrylamide-gel aegilops aegilops tauschii allelic variation amino amino acid sequence bread bread wheat breadmaking breadmaking quality capillary electrophoresis capillary-electrophoresis complete nucleotide sequence electrophoresis element enhancer gene glutenin glutenin subunit hexaploid hexaploid bread wheat its locus molecular characterization nucleotide open reading frame position primer products protein quality sds page sequence substitution subunit subunit gene synthetic hexaploid wheat tauschii triticum aestivum weight glutenin

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