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GrainGenes Reference Report: TAG-108-1299

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Reference	TAG-108-1299
Title	Similarities of omega gliadins from Triticum urartu to those encoded on chromosome 1A of hexaploid wheat and evidence for their post-translational processing
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	1299-1308
Author	DuPont FM Vensel W Encarnacao T Chan R Kasarda DD
Abstract	Summary: The omega-gliadins encoded on chromosome 1 of the A genome were purified from Triticum aestivum L. (2n=6x=42, AABBDD) cv. Butte86, nullisomic 1D-tetrasomic 1A of cv. Chinese Spring (CS N1DT1A), and the diploid T. urartu (2n=2x=14, AA). Reverse-phase high-performance liquid chromatography combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis of gliadin extracts from CS nullisomic-tetrasomic (NT) lines confirmed the assignment to chromosome 1A. The purified omega-gliadins were characterized by mass spectrometry and N-terminal sequencing. The 1A-encoded omega-gliadins were smaller than 1B- or 1D-encoded omega-gliadins. The N-terminal amino acid sequences for 1A omega-gliadin mature peptides were nearly identical to those for the T. urartu omega-gliadins and were more similar to 1D omega-gliadin sequences than to sequences for T. monococum omega-gliadins, barley C-hordeins, or rye omega-secalins. They diverged greatly from the N-terminal sequences for the 1B omega-gliadins. The data suggest that T. urartu is the A-genome donor, and that post-translational cleavage by an asparaginyl endoprotease produces those omega-gliadins with N-terminal sequences beginning with KEL
External Databases	Pubmed: 14747918
Keyword	[ Hide all but 1 of 32 ] 1b a genome amino amino acid amino acid sequence barley bread wheat c-hordein chinese chromatography chromosome chromosome-1 cleavage diploid electrophoresis genome hexaploid hexaploid wheat high performance liquid chromatography mass n-terminal sequencing nitrogen fertilization omega-gliadins rye seed storage protein sequence sodium triticum triticum aestivum triticum aestivum-l weight glutenin subunits winter wheat

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