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GrainGenes Reference Report: TAG-108-1205

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Reference	TAG-108-1205
Title	Molecular comparison of waxy null alleles in common wheat and identification of a unique null allele
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	1205-1211
Author	Saito M Konda M Vrinten P Nakamura K Nakamura T
Abstract	Summary: PCR selection markers for the identification of null waxy alleles were used to screen for waxy mutations in 168 common wheat cultivars. In all cultivars where the Wx-B1 protein was absent, the Wx-B1 allele was identical to the previously identified mutation carried by Kanto 107. Although most cultivars missing the Wx-A1 protein also carried the same Wx-A1 mutation as found in Kanto 107, all of the Turkey Wx-A1 mutants produced a different PCR fragment, implying the presence of a different mutation. Sequencing of this fragment indicated the mutation, which consisted of a 173-bp insertion in an exon, was in a different location than the previously identified Wx-A1 mutation. An 8-bp duplication of the Wx-A1 sequence flanked each end of the insertion, and an element with reverse complementary sequences was present at both ends of the insertion. These structures correspond with the features of class II transposable elements. Hence, the Turkey null Wx-A1 mutation was likely caused by the movement of a transposon, and this spontaneous mutation appears to be present in a limited geographical area
External Databases	Pubmed: 14689187
Allele	Wx-A1f (Triticum)
Germplasm	Turkey-124 Turkey-140 Turkey-171 Turkey-280 Turkey-299
Keyword	[ Hide all but 1 of 22 ] allele amylose content area common wheat dna duplication evolution exon gene insertion maize movement pcr protein retrotransposons rice selection sequence structure turkey unique waxy

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