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GrainGenes Reference Report: TAG-108-720

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Reference	TAG-108-720
Title	Evaluating the potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species
Journal	Theoretical and Applied Genetics
Year	2004
Volume	108
Pages	720-724
Author	MacRitchie D Sun G
Abstract	Summary: The potential of barley and wheat microsatellite markers for genetic analysis of Elymus trachycaulus complex species was evaluated. A set of 25 barley and 3 wheat microsatellite markers were tested for their ability to cross-amplify DNA from four accessions of E. trachycaulus and two accessions Pseudoroegneria spicata. Thirteen barley (52%) and two (68%) wheat primer pairs successfully amplified consistent products from both E. trachycaulus and P. spicata species. Four of the 15 successful primer pairs produced visible polymorphisms among the accessions tested. A higher successful rate of cross-species amplification of barley and wheat microsatellite markers in E. trachycaulus and P. spicata was found in this study. These primer pairs are now available for use as markers in genetic analysis of E. trachycaulus complex species. Our results suggest that publicly available wheat and barley microsatellite markers are a valuable resource for the genetic characterization of wild Triticeae species
External Databases	Pubmed: 14556051
Keyword	[ Hide all but 1 of 22 ] accessions amplification barley caninus complexes cross-species cross-species amplification diversity dna elymus evolution genetic-characterization hybrid isozyme locus microsatellite poaceae population products rapd triticeae wild

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