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GrainGenes Reference Report: TAG-107-1469

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Reference	TAG-107-1469
Title	Molecular studies on genetic integrity of open-pollinating species rye (Secale cereale L.) after long-term genebank maintenance
Journal	Theoretical and Applied Genetics
Year	2003
Volume	107
Pages	1469-1476
Author	Chebotar S [ Show all 6 ]
Abstract	Summary: The genetic integrity of six accessions represented by 14 sub-populations of the open-pollinating species rye (Secale cereale L.) was investigated. Seeds available from a herbarium collection (first regeneration) and from the cold store (most recent regeneration) were multiplied two to fourteen times and fingerprinted using microsatellite markers. Four accessions had significantly different allele frequencies. These were multiplied seven to thirteen times. Nearly 50% of the alleles discovered in the original samples were not found in the material present in the cold store. However alleles were detected in the most recently propagated sub-populations, that were not observed in the investigated plants of the original one. The change in allele frequencies is a continuous process. Reasons for the occurrence of genetic changes and consequences for managing open pollinating species maintained in ex situ genebanks are discussed
External Databases	Pubmed: 12898026
Keyword	[ Hide all but 1 of 22 ] accessions bread wheat cold diversity dna polymorphism fingerprinting frequencies genebank management genetic integrity germplasm analysis long-term maintenance microsatellite open-pollinating species population rapd regeneration rye secale cereale secale cereale l. triticum aestivum variability

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