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GrainGenes Reference Report: TAG-107-299

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Reference	TAG-107-299
Title	Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi
Journal	Theoretical and Applied Genetics
Year	2003
Volume	107
Pages	299-305
Author	Xia GM Xiang FN Zhou AF Wang HA Chen HM
Abstract	Summary: Suspension-derived protoplasts of Agropyron elongatum irradiated by ultra-violet light (UV) were fused with the suspension-derived protoplasts of Triticum astivum using PEG. Fertile intergeneric somatic hybrid plants were produced and various hybrid lines have been selected and propagated in successive generations. Their hybrid nature was confirmed by analysis of profiles of isozymes, RAPDs, and 5S rDNA spacer sequences, and via GISH analysis. By the procedure described, the phenotype and chromosome number of wheat could be maintained besides transfer of a few chromosomes and chromosomal fragments from the donor A. elongatum. The results above indicated that highly asymmetric fertile hybrid plants and hybrid progenies of wheat were produced via somatic hybridization
External Databases	Pubmed: 12845444
Keyword	[ Hide all but 1 of 38 ] 5s agropyron chromosome chromosome number donor elongatum fertile fertile somatic hybrid plants and progenies fragments gamma-fusion gish haynaldia villosa host hybrid hybridization irradiation isozyme isozymes light nicotiana plumbaginifolia oryza sativa phenotype progeny protoplast rapd rapds rdna regeneration resistance rice sequence somatic hybrid somatic hybridization spacer transfer triticum triticum aestivum ultra-violet light

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