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GrainGenes Reference Report: TAG-106-1184

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Reference	TAG-106-1184
Title	Development of PCR-based markers linked to dominant genes for male-fertility restoration in Pampa CMS of rye (Secale cereale L.)
Journal	Theoretical and Applied Genetics
Year	2003
Volume	106
Pages	1184-1190
Author	Stracke S [ Show all 7 ]
Abstract	Summary: Cytoplasmatic male sterility (CMS) is the basis for commercial hybrid seed production of rye. Nuclear restorer genes are indispensable for a complete restoration of fertility of the CMS lines. The drawbacks of current European restorer lines require the utilisation of new genetic resources that have been recently detected in an Iranian primitive rye population (IRAN IX) and an Argentinean landrace (Pico Gentario). The introgression of these effective restorer genes (Rfp1 and Rfp2, respectively) into breeding material can be facilitated by marker-assisted selection. Using two F2 populations based on crosses between the non-restorer inbred line Lo6 and the restorer IRAN IX, as well as Pico Gentario, RAPDs and AFLPs were screened and led to a closely linked marker set for each of these genes. The conversion of the closest markers into fragment-specific sequence-characterised amplified region (SCAR) markers resulted in flanking ranges of 2.9 cM (Rfp1) and 5.2 cM (Rfp2). The application of these markers in backcross programmes is discussed
External Databases	Pubmed: 12748768
Keyword	[ Hide all but 1 of 37 ] aflp breeding cereale l cms dna f-2 population fertility gene genetic resources genome hybrid hybrid rye hybrid seed production inbred line introgression iran isozyme landrace linkage map locus male fertility male sterility pcr-based marker rapd rapds resistance gene restoration restorer gene restorer line rflp rye scar marker secale seed seed production selection sterility

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