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GrainGenes Reference Report: TAG-106-1011

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Reference	TAG-106-1011
Title	AFLP and STS tagging of a major QTL for Fusarium head blight resistance in wheat
Journal	Theoretical and Applied Genetics
Year	2003
Volume	106
Pages	1011-1017
Author	Guo PG Bai GH Shaner GE
Abstract	Summary: Large-scale field screening for Fusarium head blight (FHB) resistance in wheat is difficult because environmental factors strongly influences the expression of resistance genes. Marker-assisted selection (MAS) may provide a powerful alternative. Conversion of amplified fragment length polymorphism (AFLP) markers into sequence-tagged site (STS) markers can generate breeder-friendly markers for MAS. In a previous study, one major quantitative trait locus (QTL) on chromosome 3BS was identified by using EcoRI-AFLP and a recombinant inbred population derived from the cross Ning 7840/Clark. Further mapping with PstI-AFLPs identified five markers that were significantly associated with the QTL. Three of them individually explained 38% to 50% of the phenotypic variation for FHB resistance. Two of them (pAGT/mCTG57, pACT/mCTG136) were linked to the QTL in coupling, and another (pAG/mCAA244) was linked to the QTL in repulsion. Successful conversion of one AFLP marker (pAG/mCAA244) yielded a co-dominant STS marker that explains about 50% of the phenotypic variation for FHB resistance in the population. The STS was validated in 14 other cultivars and is the first STS marker for a FHB resistance QTL converted from an AFLP marker
External Databases	PubMed: 12671748 https://doi.org/10.1007/s00122-002-1129-4
Germplasm	Ning 7840
Locus	Xgwm533-3B [ Show all 17 ]
Map Data	Wheat, NxC, FHB QTL
Probe	P13/M72 [ Show all 15 ]
Trait Study	FHB, Single Spikelet Inoculation, NxC, Bai03
Keyword	[ Hide all but 1 of 37 ] aflp amplified fragment length polymorphism barley blight chromosome codominant color dna marker environmental factors fhb field fragment length polymorphism fusarium fusarium graminearum fusarium head blight gene head blight locus mapping phenotypic variation polymorphism population qtl qtl tagging quantitative quantitative trait locus recombinant inbred recombinant inbred population resistance rflp scab screening selection sts trait triticum aestivum wheat scab

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