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GrainGenes Reference Report: TAG-106-644

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Reference	TAG-106-644
Title	Introgression of 1Dx5+1Dy10 into Tritordeum
Journal	Theoretical and Applied Genetics
Year	2003
Volume	106
Pages	644-648
Author	Ballesteros J [ Show all 6 ]
Abstract	Summary: The uses of hexaploid tritordeum as a crop for human consumption require improvement of its bread-making quality. For this purpose chromosome 1D of bread wheat with the Glu-D1 allele encoding for high-molecular-weight glutenin subunits Dx5+Dy10 was introgressed into tritordeum. Different primary tritordeums were crossed with wheats carrying subunits Dx5+Dy10. The hybrids were backcrossed to tritordeum and seeds for the next backcross (or selfing) were selected for the presence of chromosome 1D using SDS-PAGE. Forty two chromosome plants carrying subunits Dx5+Dy10 were obtained after two backcrosses and selfing. Chromosome characterization of these plants using fluorescence in situ hybridisation (FISH) proved that either chromosome substitution 1Hch/1D or 1A/1D had been obtained. A homozygous plant with a translocation of the entire 1DL arm to 1HchS was also obtained. The complete chromosome substitution lines have better agronomic characteristics than the lines with translocations
External Databases	Pubmed: 12595993
Keyword	[ Hide all but 1 of 34 ] agronomic characteristics allele backcross barley barley-wheat amphiploids bread bread wheat breadmaking breadmaking quality chromosome chromosome substitution crop fish fluorescence glutenin hexaploid high molecular weight high molecular weight glutenin subunits high-molecular-weight glutenin human improvement in situ in situ hybridization introgression quality repeated dna probes sds page selfing substitution substitution line translocation triticum tritordeum wheat

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