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GrainGenes Reference Report: TAG-106-353

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Reference
TAG-106-353
Title
Localisation of genes for resistance against Blumeria graminis f.sp hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp spontaneum) line
Journal
Theoretical and Applied Genetics
Year
2003
Volume
106
Pages
353-362
Author
Backes G
[ Show all 7 ]
Abstract
Summary: The aims of this investigation have been to map new (quantitative) resistance genes against powdery mildew, caused by Blumeria graminis f.sp. hordei L., and leaf rust, caused by Puccinia hordei L., in a cross between the barley (Hordeum vulgare ssp. vulgare) cultivar 'Vada' and the wild barley (Hordeum vulgare ssp. spontaneum) line '1B-87' originating from Israel. The population consisted of 121 recombinant inbred lines. Resistance against leaf rust and powdery mildew was tested on detached leaves. The leaf rust isolate 'I-80' and the powdery mildew isolate 'Va-4', respectively, were used for the infection in this experiment. Moreover, powdery mildew disease severity was observed in the field at two different epidemic stages. In addition to other DNA markers, the map included 13 RGA (resistance gene analog) loci. The structure of the data demanded a non-parametric QTL-analysis. For each of the four observations, two QTLs with very high significance were localised. QTLs for resistance against powdery mildew were detected on chromosome 1H, 2H, 3H, 4H and 7H. QTLs for resistance against leaf rust were localised on 2H and 6H. Only one QTL was common for two of the powdery mildew related traits. Three of the seven QTLs were localised at the positions of the RGA-loci. Three of the five powdery mildew related QTLs are sharing their chromosomal position with known qualitative resistance genes. All detected QTLs behaved additively. Possible sources of the distorted segregation observed, the differences between the results for the different powdery mildew related traits and the relation between qualitative and quantitative resistance are discussed
External Databases
Pubmed: 12582863
Keyword
[ Hide all but 1 of 49 ]
analog
barley
blumeria graminis
blumeria graminis f.sp hordei
chromosome
cultivar
detached leaf
difference
disease
disease resistance
dna
doubled-haploid population
f sp hordei
field
gene
graminis
hordei
hordeum
inbred line
infection
israel
leaf rust
linkage map
locus
map
mildew
population
powdery mildew
powdery mildew resistance
puccinia graminis
qtl
qtl analysis
quantitative
quantitative resistance
quantitative trait loci
recombinant inbred
recombinant inbred line
resistance
resistance gene
resistance gene analogs
rflp map
rga
rust
segregation
segregation distortion
source
vulgare ssp spontaneum
wild
wild barley

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