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GrainGenes Reference Report: TAG-106-67

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Reference	TAG-106-67
Title	Construction and analysis of a microsatellite-based database of European wheat varieties
Journal	Theoretical and Applied Genetics
Year	2002
Volume	106
Pages	67-73
Author	Roder MS [ Show all 12 ]
Abstract	Summary: A database of 502 recent European wheat varieties, mainly of winter type, was constructed using 19 wheat microsatellites and one secalin-specific marker. All datapoints were generated in at least two laboratories using different techniques for fragment analysis. An overall level of >99.5% accuracy was achieved. The 199 alleles detected allowed discrimination between all of the varieties except duplicates, and varieties derived from identical parents. Approximately 25% of the varieties showed some heterogeneities, with the highest level of heterogeneity in south-eastern European material. The highest genetic diversity and the highest number of rare alleles were found in varieties from southern Europe. The relative allele frequencies varied for most microsatellites in different geographical regions
External Databases	Pubmed: 12582872
Keyword	[ Hide all but 1 of 23 ] accuracy allele bread wheat cultivar database discrimination diversity europe fragment analysis fragment length polymorphism genetic diversity genetic diversity assessment heterogeneity heterosis maize inbreds pacific northwest parents region rflp data ssr variety identification wheat varieties winter

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