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GrainGenes Reference Report: TAG-106-1

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Reference	TAG-106-1
Title	Pyramiding transgenes for multiple resistance in rice against bacterial blight, yellow stem borer and sheath blight
Journal	Theoretical and Applied Genetics
Year	2002
Volume	106
Pages	1-8
Author	Datta K Baisakh N Maung Thet K Tu J Datta SK
Abstract	Summary: Here we describe the development of transgene-pyramided stable elite rice lines resistant to disease and insect pests by conventional crossing of two transgenic parental lines transformed independently with different genes. The Xa21 gene (resistance to bacterial blight), the Bt fusion gene (for insect resistance) and the chitinase gene (for tolerance of sheath blight) were combined in a single rice line by reciprocal crossing of two transgenic homozygous IR72 lines. F4 plant lines carrying all the genes of interest stably were identified using molecular methods. The identified lines, when exposed to infection caused by Xanthomonas oryzae pv oryzae, showed resistance to bacterial blight. Neonate larval mortality rates of yellow stem borer (Scirpophaga incertulas) in an insect bioassay of the same identified lines were 100%. The identified line pyramided with different genes to protect against yield loss showed high tolerance of sheath blight disease caused by Rhizoctonia solani
Keyword	[ Hide all but 1 of 59 ] bacillus thuringiensis bacterial bacterial blight bacterial toxins bacterial-blight bioassay blight borer chitinase chitinase gene crossing crystal proteins different genes disease disease resistance fusion gene gene transfer infection insect insect pest insect pests insect resistance ir72 mortality multiple oryza oryza sativa pest pest resistance plant breeding plant pathogenic bacteria plant pathogenic fungi plant pests pv oryzae rate resistance resistant rhizoctonia rhizoctonia solani rhizoctonia-solani rice scirpophaga incertulas sheath blight solani stem stem borer tolerance transgene transgenes transgenic transgenic plant xa21 xanthomonas xanthomonas oryzae pv xanthomonas-oryzae yellow stem borer yield yield loss

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