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GrainGenes Reference Report: TAG-105-708

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Reference	TAG-105-708
Title	Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.)
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	708-715
Author	Mano Y Komatsuda T
Abstract	Summary: Quantitative trait loci (QTLs) controlling callus growth (CG), subsequent shoot differentiation ratio (SD) and green shoot ratio (GS) in immature embryo culture were identified in barley. A base map was developed from 99 recombinant inbred lines (RILs) of 'Azumamugi' x 'Kanto Nakate Gold'. The tissue-culture traits were evaluated at the F7 and F10 generations of the RILs. The RILs showed wide and continuous variations in each of the three tissue-culture traits. Three QTLs for CG, three QTLs for SD and two QTLs for GS were detected by using composite interval mapping. A QTL for SD on chromosome 3H had a large effect, and 'Kanto Nakate Gold', which has a high differentiation ability, contributed to this QTL. The location of this QTL is identical to, or very close to, the uzu locus. We discuss the relationships between tissue-culture loci in 'Azumamugi' x 'Kanto Nakate Gold' and those in other mapping populations
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00992/paper/s00122-002-0992-3.pdf
Keyword	[ Hide all but 1 of 22 ] albinism anther culture barley callus chromosome culture differentiation embryo growth hordeum hordeum vulgare inbred line interval mapping map mapping marker quantitative trait loci recombinant inbred recombinant inbred line regeneration shoot shoot differentiation

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