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GrainGenes Reference Report: TAG-105-458

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Reference	TAG-105-458
Title	Identification of AFLP and microsatellite markers linked with an aluminium tolerance gene in barley (Hordeum vulgare L.)
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	458-464
Author	Raman H Moroni JS Sato K Read BJ Scott BJ
Abstract	Summary: Barley is the most sensitive among the cereals to aluminium (Al) stress and breeding for more tolerant cultivars is a priority. To enhance selection efficiency for Al tolerance in barley, PCR-based AFLP and microsatellite markers linked to a locus conferring tolerance to aluminium were identified. The study used F2 progeny derived from a single cross between Yambla (moderately tolerant of Al) and WB229 (tolerant of Al) and developed hydroponic pulse-recovery screening methods to assess tolerance of phenotypes based on root growth. The segregation ratios of tolerant and sensitive genotypes and F3 progeny testing suggest that a single major gene controlled Al tolerance (Alt). In order to determine the chromosomal location of the Alt gene, we used the AFLP technique coupled with bulk segregant analysis. We evaluated tolerant and sensitive bulks using 30 combinations of EcoRI/MseI primers, and 12 of these permitted differentiation of the sensitive and tolerant bulks. More than 1,000 amplified fragments were obtained, and 98 polymorphic bands were scored. AFLP analysis of wheat-barley chromosome addition lines indicated that the Alt gene was located on barley chromosome 4H. Four chromosome 4H-specific microsatellite markers (Bmac310, Bmag353, HVM68 and HVMCABG) were tightly linked to Alt. The large allelic variation detected with microsatellite marker Bmag353 allowed us to implement this marker for routine marker-assisted selection for Al tolerance, and 396 plants could be screened on a single gel
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00934/
Keyword	[ Hide all but 1 of 51 ] 30 addition addition line aflp aflp analysis al al tolerance allelic variation alp aluminium aluminium tolerance bands barley breeding bulk segregant analysis chromosomal location chromosome chromosome addition chromosome addition lines chromosome location combinations differentiation enhance fragments gene growth hordeum linkage map linkage mapping locus major gene method order polymorphic progeny ratios resistance rflp marker root rye screening screening methods segregant analysis segregation selection stress stresses tolerance tolerant cultivars triticale wheat-barley chromosome addition line

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