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GrainGenes Reference Report: TAG-105-364

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Reference	TAG-105-364
Title	A resistance gene analog useful for targeting disease resistance genes against different pathogens on group 1S chromosomes of barley, wheat and rye
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	364-368
Author	Mohler V Klahr A Wenzel G Schwarz G
Abstract	Summary: Comparative sequence analysis of the resistance gene analog (RGA) marker locus aACT/CAA (originally found to be tightly linked to the multiallelic barley Mla cluster) from genomes of barley, wheat and rye revealed a high level of relatedness among one another and showed high similarity to a various number of NBS-LRR disease resistance proteins. Using the sequence-specific polymerase chain reaction (PCR), RGA marker aACT/CAA was mapped on group 1S chromosomes of the Triticeae and was associated with disease resistance loci. In barley and rye, the marker showed linkage to orthologous powdery mildew resistance genes Mla1 and Pm17, respectively, while in wheat linkage with a QTL against fusarium head blight (FHB) disease was determined. The use of RGA clones for R gene mapping and their role in the expression of qualitative and quantitative resistance is discussed
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00919/
Keyword	[ Hide all but 1 of 47 ] aflp marker barley blight chain cluster comparative sequence analysis disease disease resistance disease resistance gene family fusarium fusarium head blight gene gene mapping group-1 head blight high-level linkage mapping marker marker locus mildew mildew resistance mildew resistance gene mla1 nbs lrr nbs-lrr pcr pm17 polymerase powdery mildew powdery mildew resistance qtl qtl analysis quantitative resistance r gene resistance resistance gene resistance gene analog rga rust resistance rye search sequence similarities targeting triticeae

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