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GrainGenes Reference Report: TAG-105-271

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Reference	TAG-105-271
Title	RFLP mapping of a Hordeum bulbosum gene highly expressed in pistils and its relationship to homoeologous loci in other Gramineae species
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	271-276
Author	Gudu S Laurie DA Kasha KJ Xia JJ Snape JW
Abstract	Summary: A cDNA sequence (Hbc8-2) isolated from pistils of the self-incompatible species Hordeum bulbosum was analysed for expression pattern and genetic map location. Hbc8-2 was expressed just prior to anthesis in mature pistils, and expression was maintained at a high level throughout anthesis. The same expression pattern was found in self-incompatible rye (Secale cereale), but no expression was detected in the self-compatible cereals wheat (Triticum aestivum) or barley (Hordeum vulgare) at comparable stages of development. However, three wheat expressed sequence tags from a pre-anthesis library had high homology to Hbc8-2. Southern blot analyses using Hbc8-2 as a probe detected hybridising bands in the genomes of various Gramineae species including rye, barley, bread wheat, wild wheat relatives (Aegilops tauschii and Ae. speltoides), oats (Avena fatua and A. strigosa), rice (Oryza sativa) and maize (Zea mays). This suggests that Hbc8-2-like sequences are present in many species but that high levels of expression may be associated with self-incompatibility. Hbc8-2 was mapped on the long arms of chromosome 2H(b) of H. bulbosum, 2R of rye, and 2B and 2D of wheat and was assigned to chromosome 2H of barley using wheat/barley addition lines. On a H. bulbosum genetic map, Xhbc8-2 was located between Xbcd266 and Xpsr87, while in rye and wheat it was located in a 13.2-cM interval between Xpsr331 and Xpsr932, consistent with previous comparative mapping studies of these species. Mapping in rye suggested that Hbc8-2 is probably proximal to the Z self-incompatibility locus which was previously shown to be tightly linked to Xbcd266
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00932/
Gene	Sr33 (Triticum) Sr9 (Triticum)
Keyword	[ Hide all but 1 of 58 ] 2d addition addition line aegilops anthesis arms avena bands barley bread cdna cdna sequence chromosome comparative mapping comparative maps expressed sequence tag gene gene expression gramineae high-level homologies homology hordeum hordeum bulbosum hordeum vulgare interval linkage map long arm maize map map location mapping mays oryza pattern phalaris coerulescens pistil powdery mildew resistance probe protein relatives rflp rflp mapping rice rye secale self-incompatibility sequence sequence tags speltoides strigosa tauschii triticum vulgare l. wheat triticum-aestivum wild wild wheat zea

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