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GrainGenes Reference Report: TAG-105-212

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Reference	TAG-105-212
Title	Cloning of cDNA for UDP-glucose pyrophosphorylase and the expression of mRNA in rice endosperm
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	212-221
Author	Abe T Niiyama H Sasahara T
Abstract	Summary: Rice endosperm UDP-glucose pyrophosphorylase (UGPase) cDNA clones were isolated by screening a lambda ZAP II library prepared from poly (A(+)) RNA of japonica rice (cv Sasanishiki) endosperm with a probe of potato UGPase cDNA. One cDNA clone, possessing about 1,700 nucleotides, contained the complete open reading frame of rice UGPase. At the nucleotide-sequence level, the UGPase cDNA of rice endosperm had high homology with the UGPase cDNA of barley endosperm (84%) and potato tuber (71%). The calculated molecular weight (50 kDa) agrees with the value determined by SDS-PAGE (51 kDa). At the amino-acid sequence level, rice UGPase has high homology with the UGPase of barley (92%) and potato (85%). The enzyme contained conserved sequence elements which are thought to be involved in substrate binding and catalytic activity. A Southern-blot analysis indicated that the gene existed as a single copy. Expression of the enzyme in rice endosperm examined by Northern-blot analysis was high at 10-15 days after heading
Keyword	[ Hide all but 1 of 36 ] amino acid amino acid sequence barley barley endosperm catalytic activity cdna cdna clone complementary dna conserved sequence endosperm enzyme gene gene expression heading homology japonica japonica rice kinase messenger rna molecular weight northern blot analysis nucleotide nucleotide sequence open reading frame oryza sativa potato probe rice rice endosperm screening sds page sequence single-copy substrate substrate-binding udpglucose pyrophosphorylase

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