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GrainGenes Reference Report: TAG-105-43

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Reference	TAG-105-43
Title	Identification of novel low M-r glutenin subunits in the high quality bread wheat cv Salmone and their effects on gluten quality
Journal	Theoretical and Applied Genetics
Year	2002
Volume	105
Pages	43-49
Author	Vaccino P [ Show all 6 ]
Abstract	Summary: Southern-blot hybridization with a probe specific for genes encoding for low M(r) glutenin subunits showed that the high quality bread wheat cv Salmone contains two DNA fragments designated as SF720 and SF750. These fragments were found to occur on the chromosome-1B satellite, and to be associated with the presence of two strongly staining low M(r) glutenin subunits in the two-dimensional A-PAGE x SDS-PAGE pattern of cv Salmone. Comparison of 65 F6 random lines derived from the cross between cv Salmone and the medium quality line FAP74809 revealed that the presence of fragments SF720 and SF750 had significant positive effects on several quality related parameters such as SDS sedimentation volume, Farinograph stability and Alveograph strength (W), tenacity (P) and elasticity (L). Additive effects of high M(r) glutenin subunits 1 and 7+9 on gluten quality were found as well. Fragments SF720 and SF750 were suggested to occur at a locus other than Glu-B3, as indicated by their relatively high frequency of recombination with the Gli-B1 locus
External Databases	http://link.springer.de/link/service/journals/00122/contents/01/00826/
Keyword	[ Hide all but 1 of 44 ] a-page additive effects allelic variation alveograph bread chromosome-1b dna dough properties durum wheat elasticity farinograph fragments frequencies gene gli-1 gliadin gluten gluten quality glutenin high-frequencies high-frequency hybridization low m-r glutenin subunits m-r making quality media organization parameters pattern probe quality random lines recombination satellite sds sds page sds sedimentation sds-sedimentation sedimentation sedimentation volume stability storage proteins subunit volume

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