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GrainGenes Reference Report: TAG-104-1362

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Reference	TAG-104-1362
Title	Flow karyotyping and chromosome sorting in bread wheat (Triticum aestivum L.)
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	1362-1372
Author	Kubalakova M Vrana J Cihalikova J Simkova H Dolezel J
Abstract	Summary: Previously, we reported on the development of procedures for chromosome analysis and sorting using flow cytometry (flow cytogenetics) in bread wheat. That study indicated the possibility of sorting large quantities of intact chromosomes, and their suitability for analysis at the molecular level. However, due to the lack of sufficient differences in size between individual chromosomes, only chromosome 3B could be sorted into a highpurity fraction. The present study aimed to identify wheat stocks that could be used to sort other chromosomes. An analysis of 58 varieties and landraces demonstrated a remarkable reproducibility and sensitivity of flow cytometry for the detection of numerical and structural chromosome changes. Changes in flow karyotype, diagnostic for the presence of the 1BL.1RS translocation, have been found and lines from which translocation chromosomes 5BL.7BL and 4AL.4AS-5BL could be sorted have been identified. Furthermore, wheat lines have been identified which can be used for sorting chromosomes 4B, 4D, 5D and 6D. The ability to sort any single arm of the hexaploid wheat karyotype, either in the form of a ditelosome or a isochromosome, has also been demonstrated. Thus, although originally considered recalcitrant, wheat seems to be suitable for the development of flow cytogenetics and the technology can be applied to the physical mapping of DNA sequences, the targeted isolation of molecular makers and the construction of chromosome- and arm-specific DNA libraries. These approaches should facilitate the analysis of the complex genome of hexaploid bread wheat
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00888/
Keyword	[ Hide all but 1 of 44 ] abilities bread chromosome chromosome analysis chromosome isolation chromosome sorting complexes cytogenetics cytometric analysis cytometry detection diagnostic difference dna dna libraries dna library flow cytogenetics flow karyotype flow-cytometry fraction genome hexaploid hexaploid bread wheat isolation karyotype karyotyping mapping marker metaphase chromosomes mitotic chromosomes physical mapping plant chromosomes quantities reproducibility root tip sensitivity sequence sorting suitability synchronization technologies translocation triticum triticum aestivum

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