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GrainGenes Reference Report: TAG-104-1164

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Reference	TAG-104-1164
Title	Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	1164-1172
Author	Guyomarc'h H Sourdille P Charmet G Edwards KJ Bernard M
Abstract	Summary: Microsatellites were isolated from a Aegilops tauschii (the D-genome donor of bread wheat) library enriched for various motifs. Primers generated from the flanking region of the microsatellites were used successfully to amplify the corresponding loci in the D genome of bread wheat. Additional amplification sometimes also occurred from the A and B genomes. The majority of the microsatellites contained (GA)n and (GT)n motifs. GA and GT repeats appeared to be both more abundant in this library and more polymorphic than other types of repeats. The allele number for both types of dinucleotide repeats fitted a Poisson distribution. Deviance analysis showed that GA and GT were more polymorphic than other motifs in bread wheat. Within each motif type (di-, tri- and tetra-nucleotide repeats), repeat number has no influence on polymorphism. The microsatellites were mapped using the Triticum aestivum Courtot x Chinese Spring mapping population. A total of 100 markers was developed on this intraspecific map, mainly on the D genome. For polyploid species, isolation of microsatellites from an ancestral diploid donor seems to be an efficient way of developing markers for the corresponding genome in the polyploid plant
External Databases	http://link.springer.de/link/service/journals/00122/contents/01/00827/paper/s00122-001-0827-7.pdf
Probe	[ Hide all but 1 of 130 ] CFD65 CFD27 CFD51 CFD53 CFD11 CFD73 CFD50 CFD35 CFD24 CFD18 CFD81 CFD8 CFD57 CFD7 CFD3 CFD49 CFD80 CFD76 CFD31 CFD14 CFD20 CFD48 CFD62 CFD44 CFD239 CFD161 CFD79 CFD223 CFD71 CFD102 CFD29 CFD188 CFD15 CFD59 CFD21 CFD61 CFD72 CFD28 CFD83 CFD58 CFD19 CFD32 CFD63 CFD2 CFD25 CFD56 CFD36 CFD77 CFD43 CFD17 CFD4 CFD55 CFD64 CFD34 CFD70 CFD9 CFD16 CFD22 CFD54 CFD39 CFD23 CFD84 CFD78 CFD67 CFD40 CFD52 CFD26 CFD12 CFD10 CFD1 CFD82 CFD30 CFD13 CFD75 CFD42 CFD33 CFD37 CFD47 CFD38 CFD5 CFD45 CFD60 CFD6 CFD74 CFD41 CFD66 CFD46 CFD69 CFD170 CFD251 CFD92 CFD282 CFD267 CFD175 CFD255 CFD160 CFD116 CFD233 CFD168 CFD270 CFD143 CFD141 CFD219 CFD152 CFD201 CFD127 CFD213 CFD211 CFD88 CFD257 CFD283 CFD106 CFD193 CFD89 CFD156 CFD165 CFD189 CFD266 CFD101 CFD183 CFD86 CFD226 CFD190 CFD135 CFD132 CFD287 CFD95 CFD242 CFD238 CFD68
Keyword	aegilops [ Show all 34 ]

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