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GrainGenes Reference Report: TAG-104-1011

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Reference	TAG-104-1011
Title	Assignment of oat linkage groups to microdissected Avena strigosa chromosomes
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	1011-1016
Author	Loarce Y Ferrer E Kunzel G Fominaya A
Abstract	Summary: Microdissection of metaphase chromosome preparations of diploid oat Avena strigosa (2n = 14) allowed isolation of the three individual chromosomes with distinct morphologies, numbers 2, 3 and 7. Using a PCR approach based on the DNA of microdissected chromosomes, STS derivatives of RFLP markers, genetically mapped in Avena spp. linkage maps, have been physically assigned to these three chromosomes. Based on either two or four RFLP-derived STS markers, the A. strigosa chromosomes 2 and 3 were found to be homoeologous to the oat linkage groups C and E, respectively. With the DNA of chromosome 7, four RFLP-derived STS markers located within the central part of linkage group F and two distal ends of linkage group G were amplified. Accordingly, chromosome 7 corresponds to linkage group F and, most probably, is involved in an A. strigosa-specific chromosomal translocation relative to the diploid species Avena atlantica and Avena hirtula, of which the cross progeny was used for linkage mapping of the tested RFLP clones
External Databases	http://link.springer.de/link/service/journals/00122/contents/02/00871/paper/s00122-002-0871-y.pdf
Keyword	[ Hide all but 1 of 40 ] a.strigosa assignment atlantica avena avena strigosa c-banded karyotypes chromosome chromosome preparation derivatives diploid diploid avena dna dna sequence ends genus avena in situ hybridization intergenomic translocations isolation linkage linkage group linkage map linkage mapping map mapping metaphase microdissected chromosomes microdissection morphology numbers oat pcr pcr markers physical mapping polymerase chain-reaction progeny rflp strigosa sts sts marker zea mays

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