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GrainGenes Reference Report: TAG-104-916

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Reference	TAG-104-916
Title	Insertional tagging of regulatory sequences in tritordeum; a hexaploid cereal species
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	916-92
Author	Salgueiro S [ Show all 9 ]
Abstract	Summary: As an approach to isolate novel cereal promoters, promoterless uidA constructs and particle bombardment were used to transform tritordeum. Five of eight transgenic lines containing uidA sequences showed evidence of promoter tagging. Expression of uidA was detected in four lines as: constitutive expression, expression in short cells of the epidermis of the spikelets, expression in pollen grains and in cells of the epidermis of the spikelet, and expression in anther primordia and pollen grains. In the fifth line, the uidA was shown by RT-PCR to be transcribed, but no GUS activity was detected. The different patterns of uidA expression indicate that different regulatory sequences were tagged in each of these lines. Analysis of the progeny resulting from self-fertilisation of the primary tagged plants, indicate that the transgenes integrated at one or two loci and the patterns of expression were stably inherited. To our knowledge, this is the first report of promoter tagging in cereals by direct gene transfer
External Databases	http://link.springer.de/link/service/journals/00122/contents/01/00836/paper/s00122-001-0836-6.pdf
Keyword	[ Hide all but 1 of 39 ] agrobacterium tumefaciens anther arabidopsis bombardment cell constitutive expression enhancer trap epidermis gene gene expression gene transfer grains gus gus activity hexaploid high-efficiency isolate knowledge locus plant development pollen primordia progeny promoter promoter tagging promoters regulatory sequence rt-pcr sequence spikelets t-dna transfer transformation transgene transgenic transgenic cereals tritordeum uida wheat-varieties

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