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GrainGenes Reference Report: TAG-104-874

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Reference
TAG-104-874
Title
Mapping of the root lesion nematode (Pratylenchus neglectus) resistance gene Rlnn1 in wheat
Journal
Theoretical and Applied Genetics
Year
2002
Volume
104
Pages
874-879
Author
[ Hide all but 1 of 6 ]
Williams KJ
Taylor SP
Bogacki P
Pallotta M
Bariana HS
Wallwork H
Abstract
Summary: The root lesion nematode, Pratylenchus neglectus, is an economically damaging pathogen of wheat and other crops. The development of P. neglectus-resistant wheat cultivars would be greatly accelerated through the use of molecular markers, as resistance phenotyping is extremely time-consuming. A greenhouse bioassay was developed to identify resistance phenotypes of doubled-haploid populations. Bulked-segregant analysis was used to identify AFLP markers linked to P. neglectus resistance in the wheat cultivar Excalibur. One resistance-linked AFLP marker was mapped close to chromosome 7A RFLP markers in a densely-mapped Cranbrook/Halberd population. One of the chromosome 7A RFLP probes, cdo347, was genotyped in the Tammin/Excalibur population segregating for response to root lesion nematode and showed 8% recombination with the P. neglectus resistance gene Rlnn1. The marker Xcdo347-7A was validated on Excalibur-and Krichauff-derived DH populations segregating for Rlnn1 and showed 14% and 10% recombination, respectively, with Rlnn1 in these populations
External Databases
http://link.springer.de/link/service/journals/00122/contents/01/00839/paper/s00122-001-0839-3.pdf
Keyword
30
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