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GrainGenes Reference Report: TAG-104-735

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Reference	TAG-104-735
Title	Utility of barley and wheat simple sequence repeat (SSR) markers for genetic analysis of Hordeum chilense and tritordeum
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	735-739
Author	Hernandez P Laurie DA Martin A Snape JW
Abstract	Summary: A selection of 36 wheat and 35 barley simple sequence repeat markers (SSRs) were studied for their utility in Hordeum chilense. Nineteen wheat and nineteen barley primer pairs amplified consistent H. chilense products. Nine wheat and two barley SSRs were polymorphic in a H. chilense mapping population, producing codominant markers that mapped to the expected homoeologous linkage groups in all but one case. Thirteen wheat and 10 barley primer pairs were suitable for studying the introgression of H. chilense into wheat because they amplified H. chilense products of distinct size. Analysis of wheat/H. chilense addition lines showed that the H. chilense products derived from the expected homoeologous linkage groups. The results showed that wheat and barley SSRs provide a valuable resource for the genetic characterization of H. chilense, tritordeums and derived introgression lines
External Databases	http://link.springer.de/link/service/journals/00122/papers/2104004/21040735.pdf
Keyword	[ Hide all but 1 of 38 ] addition addition line addition lines arbitrary primers barley chilense codominant codominant marker dna genetic analysis genetic-characterization hordeum hordeum chilense introgression introgression lines linkage linkage group linkage groups locus map mapping marker marker-assisted selection microsatellite pcr polymorphism population primer products repeat selection sequence sequence repeat markers simple sequence repeat simple sequence repeat marker ssr tritordeum utility

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