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GrainGenes Reference Report: TAG-104-720

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Reference	TAG-104-720
Title	PCR detection of Hordeum bulbosum introgressions in an H-vulgare background using a retrotransposon-like sequence
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	720-726
Author	Johnston PA Pickering RA
Abstract	Summary: Retrotransposon-like sequences are ideal tools for initial screening assays to distinguish between closely related species because of their ubiquitous presence, high copy number, chromosome coverage and rapid sequence evolution. A retrotransposon-like sequence, pSc119.1, cloned from Secale cereale (rye) has been used to obtain PCR primers that are capable of detecting small introgressions of Hordeum bulbosum (bulbous barley grass) chromatin in a Hordeum vulgare (cultivated barley) background. Combining this PCR-based assay with a crude but effective high-throughput DNA extraction has enabled the rapid identification of plants possessing H. bulbosum introgressions from large numbers of progeny from H. vulgare x H. bulbosum crosses. These plants are then further characterized by more-refined cytological, molecular and pathological techniques to locate and map the introgressed chromatin and to evaluate their disease resistance
External Databases	http://link.springer.de/link/service/journals/00122/papers/2104004/21040720.pdf
Keyword	[ Hide all but 1 of 48 ] assay assays barley chromatin chromosome copies copy number cross cultivated barley detection disease disease resistance distinguish dna dna extraction evolution extraction gene genome high-throughput hordeum hordeum bulbosum hordeum vulgare hybrid in situ hybridization introgression introgressions map molecular marker organization pcr pcr detection pcr primers powdery mildew resistance primer progeny rapid identification resistance retrotransposon-like retrotransposon-like sequence retrotransposons rye screening screening assay secale secale cereale sequence tool

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