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GrainGenes Reference Report: TAG-104-680

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Reference	TAG-104-680
Title	Identification of new low-molecular-weight glutenin subunit genes in wheat
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	680-687
Author	Ikeda TM Nagamine T Fukuoka H Yano H
Abstract	Summary: To clarify the composition of low-molecular-weight glutenin subunits (LMW-GSs) in a soft wheat cultivar, we cloned and characterized LMW-GS genes from a cDNA library and genomic DNA in Norin 61. Based on alignment of the conserved N- and C- terminal domains of the deduced amino-acid sequences, these genes are classified into 12 groups. One of these groups (group 5), the corresponding gene of which has not been reported previously, contains two additional hydrophobic amino-acid clusters interrupting the N-terminal repetitive domain. Other groups (groups 11 and 12), which were not identified in other cultivars as a protein product, showed all eight cysteines in the C-terminal conserved domain. With specific primer sets for these groups it was revealed that Glu-D3 and Glu-A3 encoded the former and the latter, respectively. Both groups of genes were expressed in immature seeds. The presence of these groups of LMW-GSs may affect the dough strength of soft wheat
External Databases	http://link.springer.de/link/service/journals/00122/papers/2104004/21040680.pdf
Keyword	[ Hide all but 1 of 42 ] alignment amino acid amino acid sequence bread wheat cdna cdna libraries cdna library cluster composition cultivar cysteine deduced amino acid sequences dna domain dough durum wheat gene genomic dna glutenin glutenin subunit group-1 chromosomes group-5 library lmw gs locus low molecular weight glutenin low molecular weight glutenin subunits low-molecular-weight glutenin subunit genes triticum aestivum low-molecular-weight glutenin subunits primer primitive wheats protein quality repetitive domain seed sequence soft soft wheat subunit subunit gene terminal domain wheat cultivar

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