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GrainGenes Reference Report: TAG-104-571

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Reference	TAG-104-571
Title	Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.)
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	571-576
Author	Iwaki K Nishida J Yanagisawa T Yoshida H Kato K
Abstract	Summary: To identify a molecular marker closely linked to Vrn-B1, the Vrn-1 ortholog on chromosome 5B, sequence polymorphism at four orthologous RFLP loci closely linked to the Vrn-1 gene family was analyzed by using near-isogenic lines of 'Triple Dirk.' At Xwg644, a RFLP locus, three types of nucleotide sequence differing by the number of (TG) repeats, two or three times, and base changes were detected. A (TG)3-type sequence proved to be specific to chromosome 5B by nulli-tetrasomic analysis, and substitution of single nucleotide (C/T) was detected between TD(B) carrying the former Vrn2 allele and TD(C) carrying the vrn2 allele. A mismatch primer was designed for dCAPS analysis of this single nucleotide polymorphism (SNP). Polymorphism was successfully detected between two NILs, through nested PCR by using a (TG)3-specific primer (1st) and a dCAPS primer (2nd) followed by a NsiI digest. The analysis of a BF2 population [(TD(B)//TD(C)] revealed the close linkage (1.7 cM) between WG644-5B and Vrn2. It was therefore concluded that the former Vrn2 locus is located on chromosome 5B and equivalent to Vrn-B1
External Databases	http://link.springer.de/link/service/journals/00122/papers/2104004/21040571.pdf
Allele	Vrn-B1a (Triticum)
Keyword	[ Hide all but 1 of 45 ] allele bread bread wheat chromosome chromosome 5a dcaps ear emergence family gene gene family genetic analysis growth habit hexaploid wheat hordeum vulgare linkage locus major genes marker molecular marker near isogenic lines near-isogenic line near-isogenic lines nested pcr nils nucleotide nucleotide sequence orthologous gene pcr plant height polymorphism population primer repeat requirement rflp sequence snp substitution synteny triticum triticum aestivum vernalization vernalization requirement vrn1 winter habit

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