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GrainGenes Reference Report: TAG-104-399

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Reference	TAG-104-399
Title	Isolation of EST-derived microsatellite markers for genotyping the A and B genomes of wheat
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	399-407
Author	Eujayl I Sorrells ME Baum M Wolters P Powell W
Abstract	Summary: Genetic variation present in 64 durum wheat accessions was investigated by using three sources of microsatellite (SSR) markers: EST-derived SSRs (EST-SSRs) and two sources of SSRs isolated from total genomic DNA. Out of 245 SSR primer pairs screened, 22 EST-SSRs and 20 genomic-derived SSRs were polymorphic and used for genotyping. The EST-SSR primers produced high quality markers, but had the lowest level of polymorphism (25%) compared to the other two sources of genomic SSR markers (53%). The 42 SSR markers detected 189 polymorphic alleles with an average number of 4.5 alleles per locus. The coefficient of similarity ranged from 0.28 to 0.70 and the estimates of similarity varied when different sources of SSR markers were used to genotype the accessions. This study showed that EST-derived SSR markers developed in bread wheat are polymorphic in durum wheat when assaying loci of the A and B genomes. A minimum of ten EST-SSRs generated a very low probability of identity (0.36x10(-12)) indicating that these SSRs have a very high discriminatory power. EST-SSR markers directly sample variation in transcribed regions of the genome, which may enhance their value in marker-assisted selection, comparative genetic analysis and for exploiting wheat genetic resources by providing a more-direct estimate of functional diversity
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/2104002/21040399.htm
Probe	DUPW4 [ Show all 22 ]
Keyword	[ Hide all but 1 of 43 ] accessions allele alleles per locus b genome bread bread wheat dinucleotide diversity dna durum durum wheat est-ssr functional diversity genetic analysis genetic resource genetic variation genome genomes genomic dna genotype genotyping hexaploid bread wheat isolation linkage map loci locus marker marker-assisted selection microsatellite microsatellite marker polymorphic polymorphism primer quality region rice selection similarities simple sequences source ssr ssr marker transcribed region

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