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GrainGenes Reference Report: TAG-104-184

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Reference	TAG-104-184
Title	Physical location of a HSP70 gene homologue on the centromere of chromosome 1B of wheat (Triticum aestivum L.)
Journal	Theoretical and Applied Genetics
Year	2002
Volume	104
Pages	184-191
Author	Francki MG Berzonsky WA Ohm HW Anderson JM
Abstract	Summary: Cereal centromeres consist of a complex organization of repetitive DNA sequences. Several repetitive DNA sequences are common amongst members of the Triticeae family, and others are unique to particular species. The organization of these repetitive elements and the abundance of other types of DNA sequences in cereal centromeres are largely unknown. In this study, we have used wheat-rye translocation lines to physically map 1BL.1RS centromeric breakpoints and molecular probes to obtain further information on the nature of other types of centromeric DNA sequences. Our results, using the rye-specific centromeric sequence, pAWRC.1, indicate that 1BL.1RS contains a small portion of the centromere from 1R of rye. Further studies used molecular markers to identify centromeric segments on wheat group-1 chromosomes. Selected RFLP markers, clustered around the centromere of wheat homoeologous group-1S chromosomes, were chosen as probes during Southern hybridization. One marker, PSR161, identified a small 1BS segment in all 1BL.1RS lines. This segment maps proximal to pAWRC.1 in 1BL.1RS and on the centromere of 1B. Sequence analysis of PSR161 showed high homology to HSP70 genes and Northern hybridization showed that this gene is constitutively expressed in leaf tissue and induced by heat shock and light stimuli. The significance of this work with respect to centromere organization and the possible significance of this HSP70 gene homologue are discussed
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/2104002/21040184.htm
Keyword	[ Hide all but 1 of 54 ] 1b 1bl.1rs centromere centromeric dna sequence centromeric sequence cereal chromosomes chromosome chromosome-1b complexes dna dna sequence drosophila-melanogaster element family gene group-1 group-1 chromosomes heat heat shock heterochromatic gene homology hsp70 hybridization in situ hybridization information light map marker member minichromosome molecular marker northern northern hybridization organization physical location probes registration repetitive dna repetitive dna sequence repetitive element rflp rflp marker rye segment sequence sequence analysis southern hybridization tissue translocation translocation line triticeae triticum triticum aestivum wheat-rye translocation

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