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GrainGenes Reference Report: TAG-103-998

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Reference	TAG-103-998
Title	High-density physical maps reveal that the dominant male-sterile gene Ms3 is located in a genomic region of low recombination in wheat and is not amenable to map-based cloning
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	998-1006
Author	Qi LL Gill BS
Abstract	Summary: In wheat it is essential to know whether a gene is located in a high or low recombination region of the genome before initiating a map-based cloning approach. The objective of this study was to explore the potential feasibility of map-based cloning of the dominant male-sterile gene Ms3 of wheat. High-density physical maps of the short arms of the group-5 chromosomes (5AS, 5BS, and 5DS) of Triticum aestivum L. were constructed by mapping 40 DNA markers on a set of 17 homozygous deletion lines. One hundred RFLP loci were mapped: 35 on 5AS, 37 on 5BS, and 28 on 5DS. A consensus physical map was colinearly aligned with a consensus genetic map of the group-5 short arms. Sixteen of the 17 markers in the consensus genetic map encompass a genetic distance of 25 cM and correspond to the distal region (FL 0.56-0.97) of the consensus physical map. Two rice probes, RG463 and RG901, previously identified to be linked to markers CDO344 and CDO749 (group-5 short arm of wheat), respectively, in the genetic map of rice chromosome 12, map between FL 0.56 and 0.63 in the consensus map. Thus at least a part of the group-5 short arm is homoeologous to a region of chromosome 12 of rice. The genetic map of chromosome arm 5AS was constructed using a population of 139 BC1 plants derived from a cross between the euploid wheat 'Chris' carrying a dominant male-sterile gene Ms3 and a disomic substitution line in which chromosome 5A of T. aestivum cv Chinese Spring was substituted by chromosome 5A from Triticum turgidum ssp. dicoccoides. The map has a genetic length of 53.4 cM with 11 DNA markers. The initial map showed that the gene Ms3 cosegregated with three markers, WG341, BCD1130 and CDO677. High-resolution mapping using an additional 509 BC1 plants indicated that the marker WG341 was closely linked to Ms3 at a genetic distance of 0.8 cM. The Ms3 was mapped physically in the region spanning 40% of the arm length from the centromere of 5AS. Therefore, map-based cloning of the Ms3 is not feasible, although WG341 can be used as a useful tag for the Ms3 gene for breeding purposes
Keyword	[ Hide all but 1 of 59 ] 5a aestivum arm barley breeding centromere chinese chinese spring chromosome chromosome 5a cloning common wheat cross deletion deletion line dicoccoides disomic substitution distance dna dna marker dominant gene genetic genetic distance genetic map genetic mapping genome group-5 high-density homoeologous group-6 chromosomes homozygous hordeum vulgare identification length line loci maize map map-based cloning mapping marker ms3 gene physical map population probes progenitor recombination region rflp rice rich regions short arms spring substitution tauschii triticum triticum aestivum turgidum wheat

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