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GrainGenes Reference Report: TAG-103-733

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Reference	TAG-103-733
Title	Characterisation of two gene subunits on the 1R chromosome of rye as orthologs of each of the Glu-1 genes of hexaploid wheat
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	733-742
Author	De Bustos A Rubio P Jouve N
Abstract	Summary: The visco-elastic properties of bread flour are firmly associated with the presence or absence of certain HMW subunits coded by the Glu-1 genes. Identifying allelic specific molecular markers (AS-PCR) associated with the presence of Glu-1 genes can serve as a valuable tool for the selection of useful genotypes. This paper reports the use of primers designed from nucleotide sequences of the Glu-D1 gene of wheat (AS-PCR for Glu-D1y10) that recognise and amplify homologous sequences of the Glu-R1 gene subunits of rye. The primers amplify the complete coding regions and provided two products of different size in rye, in wheats carrying the substitution 1R(1D) and in rye-wheat aneuploid lines carrying the long arm of chromosome 1R. The location, the molecular characterisation of these sequences and their expression during grain ripening seem to demonstrate that the amplification products correspond to structural genes encoding the high-molecular-weight (HMW) glutenins of rye. The homology of the rye gene to subunits encoding HMW glutenins in wheat was confirmed by Southern blots and sequencing. The amplification-products were cloned, sequenced and characterised, and the sequences compared with the main glutenin subunits of wheat and related species. Further, an RT-PCR experiment was performed using primers designed from the sequence of both amplified products. This assay demonstrated that both sequences are expressed in endosperm during grain ripening. The results of these analyses suggest that both gene subunits correspond to x- and y-type genes of the Glu-R1 locus of rye
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1103005/11030733.htm
Keyword	[ Hide all but 1 of 54 ] amplification aneuploid aneuploid lines as-pcr assay baking quality bread breadmaking quality chromosome endosperm flour gene genotype glu-d1 glu-d1 gene glu-r1 glutenin glutenin subunit hexaploid hexaploid wheat high molecular weight high molecular weight glutenin hmw glutenin hmw subunits homology inheritance locus long arm marker molecular marker nucleotide nucleotide sequence oct orthologous genes paper pcr analysis primer products properties region ripening rt-pcr rye secale cereale selection sequence sequencing structural gene substitution subunit tool triticum aestivum viscoelastic properties wheat

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