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GrainGenes Reference Report: TAG-103-618

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Reference	TAG-103-618
Title	AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	618-624
Author	Prins R Groenewald JZ Marais GF Snape JW Koebner RMD
Abstract	Summary: Amplified fragment length polymorphism (AFLP) markers were used to enrich the map of the wheat chromosomal region containing the Thinopyrum-derived Lr19 leaf rust resistance gene. The region closest to Lr19 was targeted through the use of deletion and recombinant lines of the translocated segment. One of the AFLP bands thus identified was converted into a sequence-tagged-site (STS) marker. This assay generated a 130-bp PCR fragment in all Lr19-carrying lines tested, except for one deletion mutant, while non-carrier template failed to amplify any product. This sequence represents the first marker to map on the distal side of Lr19 on chromosome 7el1. The conversion process of AFLP fragments to STS markers was technically difficult, mainly because of the presence of contaminating fragments. Various approaches were taken to reduce the frequency of false positives and to identify the correct clone. We were able to formulate a general verification strategy prior to clone sequencing. Various other factors causing problems with converting AFLP bands to an STS assays are also discussed
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1103004/11030618.htm
Locus	Lr19 Sr25
Probe	STSLr19-130
Keyword	[ Hide all but 1 of 41 ] aflp amplified fragment length polymorphism assay chromosome clone codominant common wheat conferring resistance conversion deletion deletion mutant fragment length polymorphism fragments gene leaf rust leaf rust resistance length polymorphism locus lr19 map marker modified forms mutant pcr polymorphism region resistance resistance gene rust rust resistance rust resistance gene segment sequence sequence tagged site sequencing strategies sts sts marker template translocation verification

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