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GrainGenes Reference Report: TAG-103-607

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Reference	TAG-103-607
Title	Molecular mapping of the reverse thermo-sensitive genic male-sterile gene (rtms1) in rice
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	607-612
Author	Jia JH [ Show all 8 ]
Abstract	Summary: TGMS (thermo-sensitive genic male-sterile) rice is widely used in hybrid rice production. Because of a specific temperature requirement, it can be used only in a narrow rice-growing zone in Asia. A newly discovered reverse thermo-sensitive genic male-sterile line, J207S, has an opposite phynotype compared to the normal TGMS lines. J207S is completely sterile when the temperature is lower than 31 degrees C. Thus, it can be widely used in a larger area. Genetic analysis indicated that the sterility of J207S was controlled by a single recessive gene which was first named as rtms1. An F2 population from the cross between J207S and E921 was developed and used for molecular mapping of the rtms1 gene. The AFLP (amplified fragment length polymorphism) technique, combined with BSA (bulked segregant analysis), was used to screen markers linked to the target gene, and eight polymorphic AFLP loci were identified. Co-segregating analysis using the F2 population showed that two of them, Rev1 and Rev7, were closely linked to the target gene with a recombinant rate of 3.8% and 7.7%, respectively. Both Rev1 and Rev7 were found to be single-copy sequences through Southern analysis. Rev1 was subsequently mapped on chromosome 10 with a doubled-haploid mapping populations derived from the cross CT9993 x IR62266 available at Texas Tech University. RM222 and RG257 were linked to Rev1 at a distance of 11.8 cM and 4.6 cM, respectively. Additional SSR markers from the rice map of Cornell University, RFLP markers from the map of RGP in Japan and the map of Texas Tech University were selected from the region surrounding Rev1 on chromosome 10 to conduct the fine-mapping of the rtms1 gene. Presently, rtms1 was mapped between RM239 and RG257 with genetic distance of 3.6 cM and 4.0 cM, respectively. The most-closely linked AFLP marker, Rev1, 4.2 cM from the rtms1 gene, was sequenced and converted into a SCAR (sequence characterized amplified region) marker which could facilitate marker-assisted selection of the rtms1 gene
Keyword	[ Hide all but 1 of 65 ] aflp aflp marker air temperature amplified fragment length polymorphism asia bsa bulked segregant analysis chromosome chromosome maps cross distance doubled-haploid environmental temperature fine mapping fine-mapping fragment length polymorphism gene genetic analysis genetic distance genetic mapping genetic markers genetic-analysis heat hybrid hybrid rice japan length polymorphism loci male sterility map mapping marker marker-assisted selection microsatellite molecular mapping oryza sativa polymorphic polymorphism population production rate recessive gene recombinant region repetitive dna requirement rflp rflp marker rice scar screen segregant analysis segregation selection sequence single-copy single-copy sequence ssr ssr marker sterility target temperature texas thermo-sensitive zone

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