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GrainGenes Reference Report: TAG-103-346

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Reference	TAG-103-346
Title	Transferability of wheat microsatellites to diploid Triticeae species carrying the A, B and D genomes
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	346-352
Author	Sourdille P Tavaud M Charmet G Bernard M
Abstract	Summary: Hexaploid wheat (Triticum aestivum L em Thell) is derived from a complex hybridization procedure involving three diploid species carrying the A, B and D genomes. In this study, we evaluated the ability of microsatellite sequences from T. aestivum to be revealed on different ancestral diploid species more or less closely related, i.e. to test for their transferability. Fifty five primer pairs, evenly distributed all over the genome, were investigated. Forty three of them mapped to single loci on the hexaploid wheat genetic map although only 20 (46%) gave single PCR products; the 23 others (54%) gave more than one band with either only one being polymorphic, the others remaining monomorphic, or with several co-segregating polymorphic bands. The other 12 detected two (9) or three (3) different loci. From the 20 primer pairs which gave one amplification product on hexaploid wheat, nine (45%) also amplified products on only one of the diploid species, and seven (35%) on more than one. Four microsatellites (20%) which mapped to chromosomes from the B genome of wheat, did not give any amplification signal on any of the diploid species. This suggests that some regions of the B genome have evolved more rapidly compared to the A or D genomes since the emergence of polyploidy, or else that the donor(s) of this B genome has(have) not yet been identified. Our results confirm that Triticum monococcum ssp. urartu and Triticum tauschii were the main donors of the A and D genomes respectively, and that Aegilops speltoides is related to the ancestor(s) of the wheat polyploid B genome
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1103002/11030346.htm
Keyword	[ Hide all but 1 of 44 ] ae.speltoides aegilops aegilops speltoides amplification b genome chromosome complexes conservation d genome emergence genetic map genome genomes hexaploid hexaploid bread wheat hexaploid wheat hybridization loci low-copy dna map marker microsatellite molecular markers nucleotide pcr pcr products phylogeny polymorphic polyploid polyploidy primer products region rflp sequence signal speltoides tauschii triticeae triticum triticum aestivum triticum monococcum triticum tauschii wheat microsatellites

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